Polyethylene Glycol Preconditioning: An Effective Strategy to Prevent Liver Ischemia Reperfusion Injury

Figure 3

Effect of PEG 35 on Akt, AMPK, eNOS activation, and NO generation. PEG 35 preconditioning at 10 mg/kg enhances AMPK and Akt activation and increases nitrite/nitrate levels without any effect on eNOS. Western blot and densitometric analysis of phosphorylated Akt/b-actin (a), phosphorylated AMPK/total AMPK (b), eNOS/b-actin (c), and biochemical determination of nitrite+nitrate levels in liver tissue (d). Data represent mean ± SEM. versus Control; versus IR 2 h.

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