The Peroxidation of Leukocytes Index Ratio Reveals the Prooxidant Effect of Green Tea Extract

Figure 2

Ratio (ratio of oxidation of the probe C11-BODIPY: FL1/FL2) of monocytes (a) and granulocytes (b), in samples collected from 10 healthy subjects before (T0) and 3 hours (T3) after the consumption of a single dose of two capsules of a green tea extract (GTE) supplement (200 mg × 2). Cells unstimulated (UNST) or treated with phorbol 12-myristate 13-acetate (PMA, 1 μg/mL), 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH, 10 mM), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox, 10 μM), Trolox + PMA, or Trolox + AAPH. Two-way (2W) RM ANOVA, with time and treatment as within-subjects factors, followed by Student-Newman-Keuls post hoc analysis: AAPH versus PMA within time: , .

(a)

(b)