Infrared Spectroscopy as a Tool to Study the Antioxidant Activity of Polyphenolic Compounds in Isolated Rat Enterocytes

Figure 3

Infrared spectra obtained using FTIRM and processed with a second derivative. (a) Average spectrums obtained with the control treatment (DMEM) during 1 hr (I) and H₂O₂-treated cells (DMEM added with 0.5 mM of H₂O₂) 30 min (II), spectra were obtained with a spectral resolution set to 6 cm⁻¹. (b) Average spectra processed with second derivative using Savitsky-Golay with 21 smoothing points and a second polynomial order. Control treatment cells (DMEM) during 1 hr (I) and H₂O₂-treated cells (DMEM added with 0.5 mM of H₂O₂) for 30 min (II). The differences seen in these treatments were analyzed by the PCA analysis to classify them and to know the most important variations in the dataset. There are two major zones of interest: proteins zone (1720–1470 cm⁻¹) and lipids zone (3200–2800 cm⁻¹).

(a)

(b)