CLOCK Promotes Endothelial Damage by Inducing Autophagy through Reactive Oxygen Species

Figure 3

CLOCK overexpression aggravated HUVECs autophagy and caused cell damage. (a) HUVECs stable overexpression CLOCK was cultured in complete medium, followed by hypoxia treatment for 48 h. (b) Numbers of LC3-positive cells from three independent experiments were shown. (c) The function of CLOCK on cell vitality was assessed by CCK-8 assay. (d) The percent of apoptotic cells in CLOCK overexpressed HUVECs were counted by flow cytometry. (e) The migrating cells in CLOCK overexpressed HUVECs were counted. (f) The expression of CLOCK, HIF-1α, LC3, and Beclin1 was measured in CLOCK overexpressed HUVECs under normoxia or hypoxia condition. , .

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