CLOCK Promotes Endothelial Damage by Inducing Autophagy through Reactive Oxygen Species

Figure 5

Involvement of ROS in CLOCK-induced cell autophagy and dysfunction under hypoxia condition. CLOCK overexpressed HUVECs were pretreated with Tiron (10 μM) for 12 h, followed by hypoxia treatment for 48 h. (a) and (b) Bar graph quantifies the relative number of autophagosomes. (c) Quantification of LC3-II fluorescent-stained cells. (d) CCK-8 assay was performed to investigate the cell vitality. (e) Flow cytometry assay was performed to detect the cell apoptosis. (f) Transwell assay was used to assess the migration capacity. (g) Western blot analysis showing the expression of CLOCK, LC3, and Beclin1. .

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