Lycium barbarum Polysaccharides Protect against Trimethyltin Chloride-Induced Apoptosis via Sonic Hedgehog and PI3K/Akt Signaling Pathways in Mouse Neuro-2a Cells

Figure 3

Effects of LBP on ROS, MDA generation, and SOD activity induced by TMT in N2a cells. Intracellular ROS was evaluated by DCFH-DA detection. Fluorescent photographs are shown after exposure to TMT (1.875, 3.75, and 7.5 μM) and 3.75 μM TMT with or without LBP (300 μg/mL) cotreatment for 24 h, scale bar: 25 μm (a). Furthermore, ROS, MDA, and SOD productions induced by TMT treatment (0, 1.875, 3.75, and 7.5 μM) alone for 24 h or 3.75 μM TMT with LBP (300 μg/mL) were quantified (b–f). The results are expressed as mean ± SD (). , compared with the control group; , compared with the TMT-treated group.

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