Lycium barbarum Polysaccharides Protect against Trimethyltin Chloride-Induced Apoptosis via Sonic Hedgehog and PI3K/Akt Signaling Pathways in Mouse Neuro-2a Cells

Figure 5

Effect of LBP on TMT-induced signaling inhibition. The cells were incubated with 300 μg/mL LBP and 3.75 μM TMT for 24 h. Fluorescent photographs are shown after exposure to TMT with or without LBP for 24 h, scale bar: 25 μm (a and b). Different protein levels expressions were determined through Western blot analysis. The results are expressed as fold increases of optical density with the internal control. The mean protein expression of control group is designated as one in the graph (c). Each bar represents mean ± SD (). , compared with the control group; , compared with the TMT-treated group.

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(b)

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