Lycium barbarum Polysaccharides Protect against Trimethyltin Chloride-Induced Apoptosis via Sonic Hedgehog and PI3K/Akt Signaling Pathways in Mouse Neuro-2a Cells

Figure 8

Effects of different antagonists on Shh and PI3K/Akt signaling pathways. Cells were pretreated with or without different inhibitors at different time points in the following concentrations: LY2940002, 10 μM; GDC-0449, 30 μM, before TMT treatment. After incubation with 3.75 μM TMT for another 24 h, the expression levels of proteins were determined through Western blot analysis. The mean protein expression of control group is designated as 1 in the graph. Each bar represents mean ± SD (). , compared with the control group; , compared with the TMT-treated group.

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