Liver Cholesterol Overload Aggravates Obstructive Cholestasis by Inducing Oxidative Stress and Premature Death in Mice

Figure 5

BDL in HC fed mice increases apoptosis. Tissue was obtained from Sham and three days after BDL animals, under (a-b) standard Chow control diet (Chow) or (c-d) high cholesterol (HC) diet, and apoptosis was assayed by (e) TUNEL immunohistochemistry; (e) quantification of positive TUNEL cells or by (f) caspase 3 activity. Images are representative from at least four independent mice. Original magnification: 100x. versus Chow fed mice, versus 3 days Chow fed mice, and versus HC fed mice.

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