Liver Cholesterol Overload Aggravates Obstructive Cholestasis by Inducing Oxidative Stress and Premature Death in Mice

Figure 6

HC diet increases oxidative stress in mice with BDL. (a) ROS content determined in fresh liver sections. Peroxides content was assayed by DCFH (5 μM) for 30 min, and superoxide content was determined by DHE (50 μM) for 30 min. (b) GSH content determined by HPLC. (c) Western blot and densitometric analysis of main oxidative stress-related proteins. Actin was used as loading control. Each column represents mean ± SEM of three independent experiments. Differences were considered significant at versus Sham Chow fed animals; versus Sham Chow fed animals at the same time. Images are representative of at least three independent experiments. Original magnification: 200x.

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