A Decrease of Brain MicroRNA-122 Level Is an Early Marker of Cerebrovascular Disease in the Stroke-Prone Spontaneously Hypertensive Rat

Figure 1

Characterization of brain expression level of microRNA-122, gp 91-phox, and NF-kB protein expression levels in JD-fed versus RD-fed SHRSR and SHRSP strains. (a) miR-122 expression level, as detected by RT-PCR, in the brains of RD- and JD-fed SHRSR and SHRSP; JD- versus RD-fed SHRSP; JD- versus RD-fed SHRSR; JD-fed SHRSR versus JD-fed SHRSP. (b) Protein expression level of gp91-phox and of NF-kB in SHRSR upon the two diets; JD- versus RD-fed SHRSR; (c) protein expression level of gp91-phox and of NF-kB in SHRSP upon the two diets. JD- versus RD-fed SHRSP. Representative Western blots and corresponding densitometric analysis are shown in panels (a) and (b). JD = high-salt Japanese style diet; RD = regular diet. for each experimental group.

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