A Decrease of Brain MicroRNA-122 Level Is an Early Marker of Cerebrovascular Disease in the Stroke-Prone Spontaneously Hypertensive Rat

Figure 7

Impact of mimic miRNA-122 overexpression on cell viability, apoptosis, and necrosis in MECs upon exposure to stress stimuli. FACS analysis (a) and corresponding percent values (b) in MECs exposed for 72 hrs to high-NaCl medium (20 mM NaCl) either in the absence or in the presence of mimic miRNA-122 overexpression. FACS analysis (c) and corresponding percent values (d) in MECs exposed for 24 hrs to medium supplemented with 200 μM H₂O₂ either in the absence or in the presence of mimic miRNA-122 overexpression. Ctr: cells transfected with lipofectamine and miRNA negative control; mimic miR-122: cells transfected with mimic miR-122; NaCl: cells exposed to high-NaCl medium for 72 hrs; H₂O₂: cells exposed to medium supplemented with 200 μM H₂O₂ for 24 hrs; NaCl + mimic miR-122: cells transfected with mimic miR-122 and exposed to high-NaCl medium for 72 hrs; H₂O₂ + mimic miR-122: cells transfected with mimic miR-122 and exposed to medium supplemented with 200 μM H₂O₂ for 24 hrs. for each comparison.

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