Oxidative Stress-Related Parthanatos of Circulating Mononuclear Leukocytes in Heart Failure

Figure 3

Linear regression analysis of the relationship between oxidative-nitrative stress, PARP activity, or AIF translocation with pro-BNP levels. (a) Linear regression of pro-BNP levels and plasma total peroxide in all subjects. (b) Linear regression of pro-BNP levels and plasma total peroxide in CHF patients. (c) Linear regression of pro-BNP and oxidative stress index in all subjects. (d) Linear regression of pro-BNP and oxidative stress index in CHF patients. (e) Linear regression of pro-BNP and leukocyte lipid peroxidation (4-hydroxynonenal (HNE)) in all subjects. (f) Linear regression of pro-BNP and leukocyte lipid peroxidation (HNE) in CHF patients. (g) Linear regression of pro-BNP and leukocyte tyrosine nitration in all subjects. (h) Linear regression of pro-BNP and leukocyte tyrosine nitration in the CHF patients. (i) Linear regression of pro-BNP and leukocyte PARyalation in all patients. (j) Linear regression of pro-BNP and leukocyte PARyalation in the CHF patients. (k) Linear regression of pro-BNP and leukocyte AIF translocation in all subjects. (l) Linear regression of pro-BNP and leukocyte AIF translocation in the CHF patients. Increased pro-BNP levels are accompanied with increased oxidative stress (PRX, OSI) and PARP activation of circulating mononuclear leukocytes either in the whole study cohort or in the CHF group alone. Lipid peroxidation, tyrosine nitration, and AIF translocation in circulating mononuclear leukocytes however show correlation with the pro-BNP levels only in the total study cohort. Continuous line represents the regression line, while dashed lines show the 95% confidence bends of the best-fit line. R²: coefficient of determination; p: level of significance.

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