Impact of Atherosclerosis- and Diabetes-Related Dicarbonyls on Vascular Endothelial Permeability: A Comparative Assessment

Figure 1

Effects of dicarbonyls and thrombin on the transendothelial electric resistance of the EA.hy926 cell monolayer. (a) Time course of dose-dependent TER changes produced by the indicated MDA concentrations and 100 nM thrombin (Thr); (b) time course of TER change produced by 250 μM MDA, GO, MGO, or glutaraldehyde (GA); (c) effects of MDA washout on the time course of TER decline produced by 200 μM MDA; (d) time course of TER change produced by 200 μM MDA in the presence of 0.5 mM NAC, lysine, or carnosine and in the presence of 2 mM lysine or carnosine. Control, cells treated with HBSS without dicarbonyls added. The arrow indicates the addition of either dicarbonyl (a–d) and thrombin (a) or MDA washout (c). TER is expressed as the normalized impedance. Data from three independent experiments were pooled. versus control, MGO, GO, or thrombin for the marked conditions at 20 hours (). ^$ versus both control and MDA at 10 hours (). ^# versus both control and MDA at 10 hours (). ^& versus MDA at 10 hours ().

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