Protective Effect of Icariin on the Development of Preimplantation Mouse Embryos against Hydrogen Peroxide-Induced Oxidative Injury

Figure 1

The effects of transient treatment of different concentrations of H₂O₂ on the development of KM mouse 1-cell embryos in vitro. Representative images of preimplantation embryos cultured under transient treatment of different concentrations of H₂O₂. Zygotes (27 h p-hCG) were pretreated with different concentrations of H₂O₂ for 30 min, and then cultured in KSOM until blastocyst (120 h p-hCG). Images of preimplantation embryos were obtained at 2-cell (45 h p-hCG), 4-cell (66 h p-hCG), and blastocyst (120 h p-hCG). In the H₂O₂-treated groups, especially the 40 μM and 60 μM groups, developmental retardation was observed. In the 80 μM H₂O₂-treated group, preimplantation embryos were arrested in 1-cell stage. Bar = 100 μm.