Triethylenetetramine Synergizes with Pharmacologic Ascorbic Acid in Hydrogen Peroxide Mediated Selective Toxicity to Breast Cancer Cell

Figure 2

Asc/TETA combination induces apoptosis in MCF-7 cells. MCF-7 cells were treated with 1 mM Asc along with 10 μM, 30 μM, or 50 μM of TETA for 12 hours. (a) MTT assay was performed to assess MCF-7 viability, , , ; (b) viability of MCF-7 cells was measured by MTT assay after 6, 12, and 24 hours of 1 mM Asc/10 M TETA treatment, . (c) Effects of different dosage of Asc/TETA (1 : 100) on proapoptotic signaling were examined by western blotting; (d) MCF-7 cells cloning formation experiments were performed after 12 hours of 1 mM Asc/10 M TETA treatment. (e) Statistic analysis of 3 independent experiments, versus control, versus Asc, .

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