Protection of Human Umbilical Vein Endothelial Cells against Oxidative Stress by MicroRNA-210

Figure 6

miR-210 downregulated CASP8AP2 pathway-related genes at the level of protein expression. (a) A Western blot showing that CASP8AP2 protein levels increased upon H₂O₂ treatment. miR-210 overexpression decreased the expression of CASP8AP2 in both H₂O₂-treated and untreated cells. (b) A Western blot showing that the cleaved caspase-8/caspase-8 ratio increased upon H₂O₂ treatment. miR-210 overexpression decreased the cleaved caspase-8/caspase-8 ratio upon H₂O₂ treatment. (c) A Western blot showing that the cleaved caspase-3/caspase-3 ratio increased upon H₂O₂ treatment. miR-210 overexpression decreased the cleaved caspase-3/caspase-3 ratio upon H₂O₂ treatment. (d) A Western blot showing that miR-210 inhibition increased CASP8AP2 protein levels in both the treated and untreated groups. (e) A Western blot showing that miR-210 inhibition increased the cleaved caspase-8/caspase-8 ratio upon H₂O₂ treatment. (f) A Western blot showing that miR-210 inhibition increased the cleaved caspase-3/caspase-3 ratio upon H₂O₂ treatment. All , .

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