MicroRNAs Modulate Oxidative Stress in Hypertension through PARP-1 Regulation

Figure 3

miR-103-2-5p and miR-585-5p target PARP-1. (a) Schematic of PARP1 coding region (CR) dual-luciferase reporter constructs. psiCHECK2 control plasmid expresses both the Renilla luciferase (RL) and Firefly luciferase (FL). Constructs of the PARP1(CR) span the indicated predicted miRNA binding sites (red color bars). (b) Cells were transfected with the dual-luciferase constructs and the indicated miRNAs or scrambled control (Scr). The ratio of RL/FL activity is shown. The histogram represents the mean + SEM from three independent experiments. by Student’s t-test compared to Scr.

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