MicroRNAs Modulate Oxidative Stress in Hypertension through PARP-1 Regulation

Figure 4

miR-103a-2-5p and miR-585-5p enhance DNA damage. HAECs were transfected with precursor mimics of miR-103a-2-5p, miR-585-5p, or scrambled control (Scr). Forty-eight hrs after transfection, cells were untreated (−) or treated with 100 μM H₂O₂ for 30 min and comet assays were performed under alkaline conditions. Representative comets are shown in (a), and olive tail moment was used as a measure of DNA damage (b). (c) Forty-eight hours after transfection with the indicated miRNAs, HAECs were untreated (−) or treated with 10 μM menadione (men) for 30 min. Cells were stained with anti-8oxoG antibodies, and fluorescent intensity was calculated as described in the Materials and Methods. The histograms represent the mean of 3 independent experiments + SEM. , , for the indicated comparisons using one-way ANOVA and Tukey’s post hoc test.

(a)

(b)

(c)