The Neuroprotective Effects of Carvacrol on Ethanol-Induced Hippocampal Neurons Impairment via the Antioxidative and Antiapoptotic Pathways

Figure 6

Carvacrol protects cultured hippocampal neurons from ethanol-induced cell death. (a)–(c) To select drug concentrations for the subsequent experiments, MTT assay was carried out on the hippocampal neurons. (a) Primary hippocampal neurons were treated with 0–300 mM ethanol. (b) Hippocampal neurons were treated with 0–1.4 mM carvacrol. (c) Hippocampal neurons were treated with 200 mM ethanol and then exposed to different doses of carvacrol. On the basis of the above results, hippocampal neurons treated with 0.8 mM carvacrol after the incubation of 200 mM ethanol were selected. (d) Hippocampal neurons were incubated with 0.8 mM carvacrol after the incubation of 200 mM ethanol. Cells were observed by fluorescence microscopy after the nuclei were stained with the fluorescent dye Hoechst 33342. All the values are presented as mean ± SEM ( per group). compared to control group; compared to ethanol group.

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