Sirt1 Protects Endothelial Cells against LPS-Induced Barrier Dysfunction

Figure 3

Protective effect of Sirt1 on LPS-induced EC hyperpermeability. (a) SRT1720 prevented LPS-induced EC hyperpermeability. HUVECs were pretreated with SRT1720 (5 μM) 24 h before (SRT1720 + LPS) or at the time (sim-SRT1720 + LPS) of LPS (500 ng/mL, 24 h) treatment; then, permeability of monolayers was measured. (b) ex527 exacerbated LPS-evoked EC hyperpermeability. HUVECs were pretreated with ex527 (20 μM) 1 h before LPS (500 ng/mL, 24 h) treatment; then, permeability of monolayers was measured. (c) Sirt1 siRNA increased LPS-evoked EC hyperpermeability. Cells treated with control or Sirt1 siRNA were exposed to LPS (500 ng/mL, 24 h); then, permeability of monolayers was measured. per group. versus control or control siRNA, ^# versus LPS or control siRNA + LPS. (d-e) The effect of SRT1720 on the distribution of F-actin and VE-cadherin. Cells were pretreated with SRT1720, followed by examining F-actin and VE-cadherin using confocal microscopy.

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