Sirt1 Protects Endothelial Cells against LPS-Induced Barrier Dysfunction

<div>The role of SRT1720 and p53 in the downregulation of <i>β</i>-catenin induced by LPS in HUVECs. (a) Time-dependent changes in expression of <i>β</i>-catenin induced by 500 ng/mL LPS. (b) Pretreatment of SRT1720 prevented LPS-induced <i>β</i>-catenin downregulation. (c) Pretreatment of ex527 aggravated LPS-induced <i>β</i>-catenin downregulation. (d) SRT1720 attenuated LPS-evoked p53 319 acetylation. (e) p53 inhibition with PFT-<i>α</i> prevented LPS-induced EC hyperpermeability. (f) p53 inhibition with PFT-<i>α</i> prevented the LPS-induced decrease in <i>β</i>-catenin expression. <svg height="8.55521pt" id="M22" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -8.34882 27.8368 8.55521" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z" id="g113-111"></path></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z" id="g117-34"></path></g><g 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LPS.</div>

Oxidative Medicine and Cellular Longevity

fig8

Figure 8

Figure 8: Sirt1 Protects Endothelial Cells against LPS-Induced Barrier Dysfunction 