Research Article
Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage
Table 1
Antibodies and their origin and dilutions used in the indirect immunofluorescence analyses.
| | Antibody | Dilution | Company | Catalog number | Secondary antibody (1 : 400) |
| | SOX17 | 10 µg/ml | R&D Systems | AF1924 | Alexa Fluor® 594 donkey anti-goat, A11058 | | HNF-4α | 1 : 50 | Santa Cruz Biotechnology | SC-6556 | Alexa Fluor 594 donkey anti-goat, A11058 | | AFP | 1 : 7000 | Dako | A0008 | Alexa Fluor 594 donkey anti-rabbit, A21207 | | CK19 | 1 : 50 | Dako | M0888 | Alexa Fluor 594 goat anti-mouse, A21125 | | EpCAM | 1 : 200 | Dako | M0804 | Alexa Fluor 594 goat anti-mouse, A21125 | | ALB | 1 : 400 | Dako | A0001 | Alexa Fluor 594 donkey anti-rabbit, A21207 | | COX IV | 1 : 100 | Abcam | Ab97770 | Alexa Fluor 594 donkey anti-rabbit, A21207 |
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