BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage

Figure 2

BAG5 reduces the levels of DJ-1, which are reversed by overexpressing Hsp70. (a) HEK293 cells stably expressing DJ-1-HA alone or with BAG5-Myc. Exogenous DJ-1 levels are obviously reduced in cells expressing BAG5-Myc comparing with cell expressing DJ-1-HA alone. Moreover, the overexpression of Hsp70-flag reversed the BAG5-mediated decrease of DJ-1-HA levels in cells stably expressing exogenous BAG5 and DJ-1. Actin was used as a loading control. (b) Quantitative data from (a) is shown. Data is normalized to DJ-1 group which was set to 1. (c) Knockdown endogenous Hsp70 accelerate reduction of ectopic DJ-1 induced by overexpression of BAG5. (d) Quantitative data from (c) is shown. The results are indicated as mean ± SEM (, , ).

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