BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage

Figure 3

BAG5 attenuated the stability of DJ-1. (a) HEK293 cells stably expressing DJ-1-HA alone, or with BAG5-Myc. All cells were treated with 1 μM CHX to inhibit total protein synthesis and cell’s extracts were analyzed by Western blot using the specified antibodies at 0 hr, 12 hr, and 24 hr, respectively. Actin acted as a loading control. In the presence of CHX, BAG5 decreased the DJ-1 levels. (b) Quantitative data from (a) showed that it reduces half-life from 24 hr to 15 hr. Data is normalized to 0 h group which was set to 100%. The results are indicated as mean ± SEM (, ).

(a)

(b)