BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage

Figure 5

BAG5 inhibited DJ-1 recruitment to mitochondria under rotenone treatment. HEK293 cells stably expressed DJ-1-HA alone (a, c) or with BAG5-Myc (b, d). DJ-1 and mitochondria were stained by HA (red) and Tom20 (green) antibodies, respectively. Without rotenone treatment, exogenous DJ-1-HA (green) has almost no localization on mitochondria marker Tom20 (red) in cells stably expressing vector (a) or BAG5-Myc (b). After being treated with rotenone, increased mitochondrial localization of DJ-1 was observed in cells stably expressing DJ-1-HA alone (c) comparing with BAG5-Myc + DJ-1-HA (d). The enlarged area is displayed as a white box. Quantitative analysis of colocalization is shown (e). Results are shown as mean ± SEM ().

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