Characterization of NO-Induced Nitrosative Status in Human Placenta from Pregnant Women with Gestational Diabetes Mellitus

Figure 3

S-Nitrosylation and activation level in AKT and ERK1/2. Notes. ((a) and (c)) ERK1/2 (a) and AKT (c) phosphorylation quantified by Western blotting in control and GDM placental tissue. Results are expressed as means ± SEM of phosphoprotein to total-protein ratio measured by densitometry . Lower panels are representative images of immunoblotted proteins. ((b) and (d)) S-Nitrosylation measured in placental tissue obtained from control and GDM pregnancies. Methods used were biotin-switch technique in ERK1/2 (b) and AKT (d). Results are presented in bar charts as mean ± SEM of SNO-protein (). Lower panels are representative images of immunoblotted SNO-proteins. Units on -axes of bar charts are arbitrary. , GDM versus control; , GDM versus control.

(a)

(b)

(c)

(d)