Homocysteine Induces Apoptosis of Human Umbilical Vein Endothelial Cells via Mitochondrial Dysfunction and Endoplasmic Reticulum Stress

Figure 2

Hcy induced the expression of NOX4 and production of ROS in HUVEC in a time-dependent manner. (a) The expression levels of NOX4 were determined via Western blot analysis. β-actin was selected as the loading control. (b) The production of ROS in the mitochondria and cytoplasm was detected via MitoSOX Red mitochondrial superoxide indicator and DCFH-DA staining. The data are presented as the means ± SD of three independent experiments. , versus the 0-hour group.

(a)

(b)