Impact on Autophagy and Ultraviolet B Induced Responses of Treatment with the MTOR Inhibitors Rapamycin, Everolimus, Torin 1, and pp242 in Human Keratinocytes

<div>HaCaT cells were treated with or without 50 mJ/cm<sup>2</sup> UVB and then incubated in the presence or absence of 20 nM Rapamycin (a), 100 nM everolimus (b), 1 <i>μ</i>M Torin 1 (c), or 1 <i>μ</i>M pp242 (d) for 12 hours. Western blotting was performed using primary antibodies against MTOR, phospho-Ser2448 or Ser2481 MTOR, p70 S6 kinase, phospho-Thr389 or Ser371 p70 S6 kinase, S6 ribosomal protein, phospho-Ser240/244 or Ser235/236 S6 ribosomal protein, 4E-BP1, phospho-Thr37/46 or Ser65 4E-BP1, ULK1, phospho-Ser555 or Ser757 ULK1, Rictor, phospho-Thr1135 Rictor, SGK1, phospho-Ser78 SGK1, Akt, and phospho-Ser473 Akt. GAPDH served as a loading control. Representative figures were exhibited from three independent experiments.</div>

Oxidative Medicine and Cellular Longevity

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Figure 4

Figure 4: Impact on Autophagy and Ultraviolet B Induced Responses of Treatment with the MTOR Inhibitors Rapamycin, Everolimus, Torin 1, and pp242 in Human Keratinocytes 

Figure 4 | Impact on Autophagy and Ultraviolet B Induced Responses of Treatment with the MTOR Inhibitors Rapamycin, Everolimus, Torin 1, and pp242 in Human Keratinocytes 