Impact on Autophagy and Ultraviolet B Induced Responses of Treatment with the MTOR Inhibitors Rapamycin, Everolimus, Torin 1, and pp242 in Human Keratinocytes

<div>HaCaT cells were treated with or without 50 mJ/cm2 UVB and then incubated in the presence or absence of 20 nM Rapamycin (a), 100 nM everolimus (b), 1 μM Torin 1 (c), or 1 μM pp242 (d) for 12 hours. Western blotting analysis was performed using primary antibodies against phospho-Ser428 ATR, phospho-Ser1981 ATM, phospho-Ser139 H2A X, Calnexin, BiP, PDI, phospho-Thr980 PERK, PERK, phospho-Ser724 IRE1α, IRE1α, phospho-Ser51 eIF2α, CHOP, phospho-Thr183/185 SAPK/JNK, and SAPK/JNK. GAPDH served as a loading control. Representative figures were exhibited from three independent experiments.</div>

Oxidative Medicine and Cellular Longevity

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Figure 5

Figure 5: Impact on Autophagy and Ultraviolet B Induced Responses of Treatment with the MTOR Inhibitors Rapamycin, Everolimus, Torin 1, and pp242 in Human Keratinocytes 

Figure 5 | Impact on Autophagy and Ultraviolet B Induced Responses of Treatment with the MTOR Inhibitors Rapamycin, Everolimus, Torin 1, and pp242 in Human Keratinocytes 