Immediate Early Response Gene X-1 (IEX-1) Mediates Ischemic Preconditioning-Induced Cardioprotection in Rats

Figure 6

IEX-1 overexpression inhibits H/R-induced ROS accumulation. Neonatal rat cardiomyocytes were infected with adenovirus at 10 MOI for 36 h, then underwent H/R (4 h/30 min). (a) ROS levels measured by luminol chemiluminescence (left). The column data are the sum counts of the 30-minute measurement period (right). (b) Intracellular ROS were detected by DCFH-DA (scale bar = 40 μm), and (c) mitochondrial ROS were detected by MitoTracker Red CM-H2XRos probe with confocal microscopy (scale bar = 20 μm). versus Ctrl, ^# versus GFP or β-Gal.

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