Effect of Cocoa Polyphenolic Extract on Macrophage Polarization from Proinflammatory M1 to Anti-Inflammatory M2 State

Table 2

Identification by HPLC-PDA-MS of cocoa polyphenolic bioactive molecule extract. UV maximum absorption (UV), retention times (t_R), and m/z ([M-H]−) values of polyphenols identified in cocoa extract were analyzed.

Peak number	Compound	UV	tR	m/z [M-H]−
1	Unknown	272	1.7
2	Caffein	272	2.0	195
3	Unknown	210, 270	2.2	387, 453
4	Chrysophanol-hexoside	210, 274	3.3	415, 253
5	Quinic acid	298, 320	3.6	191
6	Vanillic acid derivative	220, 275	4.7	282
7	Unknown	296	5.1	449
8	Theobromina	272	7.5	181
9	Protocatechuic acid	228, 260, 294	8.0	153
10	Unknown	467	9.2	467
11	Cinnamic acid derivative	213, 301, 320	11.2	294
12	Catechin-3-O-glucoside	278	11.4	451, 289
13	Catechin derivate	278	12.0	497, 451
14	Catechin sulphonic acid	274	12.6	369, 289
15	Unknown	294, 298, 307	13.6	407, 305
16	Procyanidin B dimer	278	14.4	577
17	Procyanidin B dimer	278	15.5	577
18	Procyanidin trimer	278	17	865
19	Procyanidin tetramer	278	17.5	1153
20	Clovamide	320	17.9	358
21	Catechin derivative	278	18.6	720
22	Procyanidin B dimer	278	21.5	577
23	Dideoxyclovamide	320	23.8	326, 282