The Repeated Administration of Resveratrol Has Measurable Effects on Circulating T-Cell Subsets in Humans

Figure 5

The effects of resveratrol on the in vitro proliferation of human Treg cells. PBMCs from healthy donors were cultured for seven days in T-cell medium stimulated with anti-CD3/CD28 beads to activate TCR in the presence of the indicated concentrations of resveratrol. (a) A representative flow cytometry result showing the percentage of CD3⁺CD4⁺CD25⁺CD127⁻ cTreg cells. (b) A summary of the flow cytometry data showing the percentage of CD3⁺CD4⁺CD25⁺CD127⁻ cTreg cells derived from three healthy individuals. (c) PBMCs from two healthy donors were cultured with anti-CD3/CD28 beads as positive control or with various concentrations of resveratrol (resv) but without anti-CD3/CD28 beads. The percentage of CD3⁺CD4⁺CD25⁺CD127⁻ cTreg cells was assessed in the same manner as (a). (d) PBMCs from three healthy donors were cultured for seven days in T-cell medium stimulated with anti-CD3/CD28 beads in the presence of the indicated concentrations of resveratrol. Flow cytometry analysis was performed to enumerate the percentage of Treg cells, which were gated as live⁺CD3⁺CD4⁺Foxp3⁺Treg cells. A representative flow cytometry result is shown. (e) A summary of the flow cytometry data showing the percentage of CD3⁺CD4⁺Foxp3⁺Treg cells derived from three healthy individuals. .

(a)

(b)

(c)

(d)

(e)