Nicotinamide Adenine Dinucleotide Protects against Spinal Cord Ischemia Reperfusion Injury-Induced Apoptosis by Blocking Autophagy

Figure 2

Immunofluorescence staining of LC3B in sham, ischemia reperfusion (I/R), 10 mg/kg nicotinamide adenine dinucleotide (NAD⁺), and 75 mg/kg NAD⁺ group. (a) LC3B-positive neurons (arrow) were observed in different groups (400x, scale bar = 50 μm). (b) Analysis of fluorescence intensity of LC3B-positive neurons in each group. Compared with the sham group, the fluorescence intensity of LC3B-positive neurons was significantly increased in the I/R group. Compared with the I/R group, the fluorescence intensity of LC3B-positive neurons was significantly decreased in the 75 mg/kg NAD⁺ group. There was no significant difference in 10 mg/kg NAD⁺ group. Values are means ± SD ( per group). versus sham; versus I/R; versus I/R.

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