Nicotinamide Adenine Dinucleotide Protects against Spinal Cord Ischemia Reperfusion Injury-Induced Apoptosis by Blocking Autophagy

Figure 4

Nissl staining in sham, ischemia reperfusion (I/R), 10 mg/kg NAD⁺, and 75 mg/kg nicotinamide adenine dinucleotide (NAD⁺) group. (a) Nissl bodies were observed in different groups (100x, scale bar = 100 μm). (b) Analysis of the mean integrated option density (IOD) of Nissl bodies in each group. Compared with the sham group, the mean IOD of Nissl bodies was significantly decreased in the I/R group. Compared with the I/R group, the mean IOD of Nissl bodies was significantly increased in the 75 mg/kg NAD⁺ group. No significant differences were observed in the 10 mg/kg NAD⁺ group. Values are means ± SD ( per group). versus sham; versus I/R; versus I/R.

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