Nicotinamide Adenine Dinucleotide Protects against Spinal Cord Ischemia Reperfusion Injury-Induced Apoptosis by Blocking Autophagy

Figure 6

Immunofluorescence and immunohistochemical staining of Bax in sham, ischemia reperfusion (I/R), 10 mg/kg nicotinamide adenine dinucleotide (NAD⁺), and 75 mg/kg NAD⁺ group. (a) Bax-positive neurons (arrow) were observed in different groups (200x, scale bar = 100 μm). (b) Bax-positive neurons (arrow) were observed in different groups (100x, scale bar = 100 μm). (c, d) Analysis of fluorescence intensity of Bax-positive neurons and the mean integrated option density (IOD) of Bax in each group, respectively. Compared with the sham group, the fluorescence intensity of Bax-positive neurons and the mean IOD of Bax were significantly increased in the I/R group. Compared with the I/R group, the fluorescence intensity of Bax-positive neurons and the mean (IOD) of Bax-positive neurons were significantly decreased in the 75 mg/kg NAD⁺ group and were not significantly different in the 10 mg/kg NAD⁺ group. Values are means ± SD ( per group). versus sham; versus I/R; versus I/R.

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