REGγ Contributes to Regulation of Hemoglobin and Hemoglobin δ Subunit

Figure 4

Regulation of endogenous HBD in HeLa cells. (a) HeLa cells were transfected with HA-pSG5-HBD plasmid (1 μg/ml) and HA-Smurf1 (1 μg/ml) as a positive control, using Lipo2000 transfection reagent for 72 h incubation. Total protein extracts were analyzed by Western blot against anti-HA antibody. (b) CHX assay for endogenous degradation. HeLa cells transfected with HA-pSG5-HBD. After 64 h incubation, cells were treated 100 μg/ml of CHX for 0, 2, 4, 6, and 8 h. The total protein extracts were subjected to Western blot analysis. β-Actin was used as loading control. (c) Quantification of CHX-treated Western blot results expressed as the means ± SEM. ; versus control.

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