REGγ Contributes to Regulation of Hemoglobin and Hemoglobin δ Subunit

Figure 5

REGγ-mediated degradation of HBD in HeLa cells (a). Cells were transiently transfected with HA-pSG5-HBD (2 μg), FRT-REGγwt (1 μg), FRT-REGγN151Y (1 μg), HA-pSG5 vector (2 μg), and FRT vector (1 μg) using Lipo2000 transfection reagent for 72 h incubation. Protein expression was detected against anti-REGγ, anti-HBD, and anti-β-actin antibodies. Nontransfected HeLa cells were used as a control. (b) HeLa cells were transfected with pcDNA3.1-p21 (2 μg) FRT-REGγwt (1 μg), FRT-REGγN151Y (1 μg), pcDNA3.1 vector (2 μg), and FRT vector (1 μg) using Lipo2000 transfection reagent for 72 h incubation. Protein expressions of indicated antibodies were determined by Western blot analysis. Nontransfected HeLa cells were used as controls. β-Actin was used as loading control. The quantification analysis was conducted and shown as a graph. Left: Western blot. Right: quantification analysis. Data is presented as means ± SEM. ; versus control.

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(b)