Acarbose Accelerates Wound Healing via Akt/eNOS Signaling in db/db Mice

Figure 4

Acarbose therapy improved BM-EPC function and decreased intracellular reactive oxygen species (ROS) levels in db/db mice. (a) Circulating EPC numbers were detected by flow cytometry and the percentage of Sca-1⁺/Flk-1⁺ cells was calculated. Acarbose significantly increased circulating EPC number in db/db mice. (b) Typical images of tube formation assay of BM-EPCs. The number of tubes in each sample was calculated from 5 low-power fields (50x; scale bar = 100 μm) at random. Acarbose enhanced the capacity of tube formation of BM-EPCs. (c) Intracellular NO level was determined by flow cytometry and the percentage of DAF fluorescence intensity was calculated. Acarbose obviously enhanced NO level in BM-EPCs. (d) DHE fluorescence intensity was determined by flow cytometry. Acarbose suppressed intracellular level in BM-EPCs. , , and versus Con; , , and versus db/db. Values are expressed as the mean ± standard deviation (–9 per group). Con, control; Aca, acarbose.

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