Mulberry Anthocyanin Extract Ameliorates Oxidative Damage in HepG2 Cells and Prolongs the Lifespan of Caenorhabditis elegans through MAPK and Nrf2 Pathways

Figure 4

MAE treatment altered expressions of proteins associated with oxidative stress. (a) Protein levels of Nrf2, HO-1, PGC-1α, and FOXO1 in total cell extracts. Equal loading of Western blots was ensured by β-actin. (b) Protein levels of phosphorylated and total JNK and p38 in total cell extracts. Results were expressed as the ratio of phosphorylated/total protein levels. (c) GSH and (d) MDA contents determined by commercially available kits (NJJCBIO, China). Vertical lines represent standard deviations of three replicates. Values with different letters above are significantly different, , one-way ANOVA test.

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