Cardioprotective Effects of Transfusion of Late-Phase Preconditioned Plasma May Be Induced by Activating the Reperfusion Injury Salvage Kinase Pathway but Not the Survivor Activating Factor Enhancement Pathway in Rats

Figure 2

Phosphorylation of STAT3, Akt, and Erk1/2 in normal myocardium obtained after fluid transfusion but prior to myocardial ischemia reperfusion. Normal myocardium of recipient rats was collected at 24 hours after fluid transfusion. Equal amounts of protein lysate from the normal myocardium of recipient rats receiving nonpreconditioned plasma transfusion (group NPP), normal saline (group NS), or preconditioned plasma (group PP) were subjected to Western blot ( per group). Error bars represent the means ± SD. (a) Representative images of Western blots. Phosphorylated STAT3 (p-STAT3) and STAT3, phosphorylated Akt (p-Akt) and Akt, phosphorylated Erk1/2 (p-Erk1/2) and Erk1/2, and GAPDH are shown. Western blot bands of p-STAT3 were not observed. GAPDH was used as a loading control. (b) Phosphorylation levels of Akt, expressed as the ratio of p-Akt to Akt. (c) Phosphorylation level of Erk1/2, expressed as the ratio of p-Erk1/2 to Erk1/2. versus group NPP; ^# versus group NS.

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