Selaginella bryopteris Aqueous Extract Improves Stability and Function of Cryopreserved Human Mesenchymal Stem Cells

Figure 5

Analysis of cytoskeleton distribution in postthawed MSCs by confocal microscopy: (a) cytoskeleton distribution in noncryopreserved MSCs after 48 h of culture; (b) cytoskeleton distribution in noncryopreserved MSCs after 72 h of culture; (c) cytoskeleton distribution in postthawed MSCs cryopreserved with Selaginella bryopteris water extract (WS) after 48 h of culture; (d) cytoskeleton distribution in postthawed MSCs cryopreserved with Selaginella bryopteris water extract (WS) after 72 h of culture; (e) cytoskeleton distribution in postthawed MSCs cryopreserved with Me₂SO after 48 h of culture; (f) cytoskeleton distribution in postthawed MSCs cryopreserved with Me₂SO after 72 h of culture; (g) cytoskeleton distribution in postthawed MSCs cryopreserved with WS + Me₂SO after 48 h of culture; (h) cytoskeleton distribution in postthawed MSCs cryopreserved with WS + Me₂SO after 72 h of culture.

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