The Protective Effect of Indole-3-Acetic Acid (IAA) on H<sub>2</sub>O<sub>2</sub>-Damaged Human Dental Pulp Stem Cells Is Mediated by the AKT Pathway and Involves Increased Expression of the Transcription Factor Nuclear Factor-Erythroid 2-Related Factor 2 (Nrf2) and Its Downstream Target Heme Oxygenase 1 (HO-1)

<div>IAA treatment suppresses H<sub>2</sub>O<sub>2</sub>-induced cytotoxicity via regulation of the cell cycle and apoptosis in human dental pulp stem cells (hDPSCs). (a) Analysis of cell cycle after IAA treatment of H<sub>2</sub>O<sub>2</sub>-damaged hDPSCs. (b) DNA staining with DAPI. The number of cells with DNA condensation (arrow) decreased after IAA treatment of H<sub>2</sub>O<sub>2</sub>-damaged hDPSCs. Expression of the apoptosis-related genes <i>BAX</i>, <i>p53</i>, <i>BCL-2</i>, and <i>ATF5</i> was evaluated by real-time PCR (c) and the expression of the apoptosis-related enzyme, cleaved caspase-3, was evaluated by Western blot (d). <svg height="8.8423pt" id="M8" style="vertical-align:-0.2064009pt" version="1.1" viewbox="-0.0498162 -8.6359 32.2343 8.8423" width="32.2343pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M822 650H589L583 622C660 617 677 607 674 561C672 534 664 481 647 390L600 137H596L273 650H126L120 622C176 620 194 615 207 594C221 571 225 557 214 504L161 257C141 166 129 112 121 85C108 42 83 30 29 28L23 0H260L266 28C193 33 173 42 176 89C178 122 186 172 202 255L256 527H259L583 -8H612L690 390C708 481 720 535 728 558C744 603 756 619 816 622L822 650Z" id="g113-79"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="839" vert-adv-y="839" xpluscharwidth="10.907"></glyph.data></g><g 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Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: The Protective Effect of Indole-3-Acetic Acid (IAA) on H<sub>2</sub>O<sub>2</sub>-Damaged Human Dental Pulp Stem Cells Is Mediated by the AKT Pathway and Involves Increased Expression of the Transcription Factor Nuclear Factor-Erythroid 2-Related Factor 2 (Nrf2) and Its Downstream Target Heme Oxygenase 1 (HO-1) 

Figure 4 | The Protective Effect of Indole-3-Acetic Acid (IAA) on H2O2-Damaged Human Dental Pulp Stem Cells Is Mediated by the AKT Pathway and Involves Increased Expression of the Transcription Factor Nuclear Factor-Erythroid 2-Related Factor 2 (Nrf2) and Its Downstream Target Heme Oxygenase 1 (HO-1) 