The Protective Effect of Indole-3-Acetic Acid (IAA) on H₂O₂-Damaged Human Dental Pulp Stem Cells Is Mediated by the AKT Pathway and Involves Increased Expression of the Transcription Factor Nuclear Factor-Erythroid 2-Related Factor 2 (Nrf2) and Its Downstream Target Heme Oxygenase 1 (HO-1)

Figure 6

Effects of IAA on nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase 1 (HO-1) and the role of AKT signaling in human dental pulp stem cells (hDPSCs). (a) The expression of Nrf2 was analyzed in cytosolic (Cy) and nuclear (Nu) extracts from cells. The antioxidant enzyme, HO-1, was also profiled after treatment of H₂O₂-damaged hDPSCs with IAA. (b) Densitometric analysis of Cy-Nrf2, Nu-Nrf2, and HO-1 expression. (c) Roles of AKT signaling in IAA-induced Nu-Nrf2 and HO-1 expression. The IAA-induced increase in expression of pAKT, Nu-Nrf2, and HO-1 was significantly reduced by treatment with the AKT inhibitor LY294002. (d) Densitometric analysis of pAKT, Nu-Nrf2, and HO-1 expression. , .

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