Deficiency of the Purinergic Receptor 2X₇ Attenuates Nonalcoholic Steatohepatitis Induced by High-Fat Diet: Possible Role of the NLRP3 Inflammasome

Table 2

Liver inflammation, fibrosis, and lipid metabolism. Liver expression of genes of inflammation, fibrosis, and lipid metabolism in WT and Pr2x₇^−/− mice fed with a NFD or a HFD (mean ± SD; per group). versus the corresponding NFD mice; versus the corresponding WT mice.

	WT-NFD	Pr2x7^−/−-NFD	WT-HFD	Pr2x7^−/−-HFD
Inflammation
Ccl2	0.90 ± 0.30	0.98 ± 0.28	2.79 ± 0.79	1.56 ± 0.49^‡
Tnfa	1.07 ± 0.22	1.03 ± 0.58	2.18 ± 0.71^†	1.13 ± 0.31^§
Ifng	1.02 ± 0.13	1.03 ± 0.06	1.27 ± 0.20	1.18 ± 0.11
Cxcr3	0.97 ± 0.13	1.06 ± 0.26	2.86 ± 1.07	1.65 ± 0.50^#
Adgre1	1.08 ± 0.26	1.11 ± 0.55	2.54 ± 0.60	1.62 ± 0.37^§
Ddit3	1.01 ± 0.07	0.98 ± 0.08	1.93 ± 0.20	1.17 ± 0.30^‡
Ager	1.02 ± 0.07	1.03 ± 0.07	1.93 ± 0.18	1.28 ± 0.38^§
Fibrosis
Fn1	0.98 ± 0.06	0.92 ± 0.07	1.68 ± 0.29	1.09 ± 0.03^‡
Cola1a1	1.00 ± 0.17	0.93 ± 0.06	2.33 ± 0.49	1.34 ± 0.27^§
Tgfβ	1.08 ± 0.13	1.01 ± 0.22	1.84 ± 0.26	1.50 ± 0.26^†#
Lipid metabolism
Srebf1	0.91 ± 0.15	0.99 ± 0.22	3.80 ± 0.52	1.90 ± 0.27^‡
Ppara	0.97 ± 0.21	0.93 ± 0.07	1.90 ± 0.52^†	1.45 ± 0.33
Pparg	0.92 ± 0.16	0.93 ± 0.15	2.52 ± 0.71	1.47 ± 0.13^§
Nr1h3	0.96 ± 0.09	0.92 ± 0.12	1.47 ± 0.14	1.24 ± 0.10^†#
Nr1h2	1.09 ± 0.12	1.12 ± 0.14	1.22 ± 0.21	1.23 ± 0.19
Acaca	0.87 ± 0.15	0.92 ± 0.11	1.21 ± 0.06	0.97 ± 0.08^‡
Fasn	0.96 ± 0.15	0.99 ± 0.20	1.53 ± 0.11	1.19 ± 0.17^§
Cpt1	0.95 ± 0.09	1.02 ± 0.19	2.45 ± 0.39	1.64 ± 0.28^‡
Acox1	0.95 ± 0.08	0.90 ± 0.14	1.07 ± 0.26	1.00 ± 0.22
Hmgcr	0.99 ± 0.05	0.98 ± 0.07	1.10 ± 0.16	1.05 ± 0.16
Mttp	0.91 ± 0.10	0.98 ± 0.08	0.91 ± ±0.18	0.99 ± 0.23

WT = wild type; Pr2x₇^−/− = knockout for purinergic receptor X2₇ gene; NFD = normal-fat diet; HFD = high-fat diet; Ccl2 = monocyte chemoattractant protein-1 (MCP-1) gene; Tnfa = tumor necrosis factor-α gene; Ifng = interferon-γ gene; Cxcr3 = CX chemokine receptor 3 gene; Adgre1 = F4/80 gene; Ddit3 = m CCAAT/enhancer-binding protein (C/EBP) homologous protein (CHOP) gene; Ager = receptor for AGEs (RAGE) gene; Fn1 fibronectin gene; Col1a1 = collagen I gene; tgfb1 = transforming growth factor- (TGF-) β1 gene; Srebf1 = sterol regulatory element-binding transcription factor 1c gene; Ppara = peroxisome proliferator-activated receptor (PPAR) α gene; Pparg = peroxisome proliferator-activated receptor (PPAR) γ gene; Nr1h3 = liver X receptor- (LRX-) α gene; Nr1h2 = LXR-β gene; Acaca = acetyl-CoA carboxylase gene; Fasn = fatty acid synthase gene; Cpt1a = carnitine palmitoyltransferase- (CPT-) I gene; Acox1 = acyl-CoA oxidase 1 gene; Hmgcr = hydroxymethylglutaryl-CoA reductase gene; Mttp = microsomal triglyceride transfer protein gene. or versus the corresponding NFD-fed mice; , , or ^#, versus WT mice.