Differential Alterations of the Mitochondrial Morphology and Respiratory Chain Complexes during Postnatal Development of the Mouse Lung

Figure 1

Western blot analyses of distinct mitochondrial proteins in lung homogenates from newborn, P15, and adult mice. (a) Following the homogenization, 50 μg of protein isolated from the lungs of each group (NB, P15, and adult) was resolved by 10% SDS-polyacrylamide gel electrophoresis, and the blots were immunostained with anti-MT-ND1, anti-SDHD, anti-COX1 (OXPHOS complex IV subunit I), anti-COX2 (OXPHOS complex IV subunit II), anti-ATP synthase (ATP5b), and anti-POLG2. GAPDH was used as a loading control. Nuclear-encoded protein. Mitochondrially encoded protein. (b) Bar graphs summarizing normalized data. values were calculated by the one-way ANOVA using Tukey’s test. ; , , , and .

(a)

(b)