Differential Alterations of the Mitochondrial Morphology and Respiratory Chain Complexes during Postnatal Development of the Mouse Lung

Figure 5

mRNA expression of the mtDNA transcription machinery genes, mitochondrially encoded RNA, and mitochondrial DNA polymerase in the new born, P15, and adult lung tissues as determined via quantitative real-time (qRT-PCR) analysis. Total RNA was extracted from the lung tissues of newborn (NB), P15, and adult (AL) mice using RNAzol, reverse transcribed and subjected to qRT-PCR using specific primers of the mtDNA transcription machinery (Polrmt, Tfam, Tfb1m, and Tfb2m), mitochondrially encoded RNAs (mt-Rnr1 and mt-Rnr2), and mitochondrial DNA polymerase (Polg2) involved in mitochondrial DNA replication. The bar graphs represent relative levels of the transcripts in three independent experiments. Statistical significance is indicated by , , and . The mRNA levels were normalized to the ones of GAPDH.

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