Research Article
Effect of ALDH2 on High Glucose-Induced Cardiac Fibroblast Oxidative Stress, Apoptosis, and Fibrosis
Table 1
Quantitative polymerase chain reaction primers for ALDH2, collagen I, collagen III, and GAPDH.
| | Gene (accession number) | Sequence | Annealing temperature (°C) | Product (bp) |
| ALDH2
(NM_032416.1) | Forward 5-GTG TTC GGA GAC GTC AAA GA-3
Reverse 5-GCA GAG CTT GGG ACA GGT AA-3 | 62.5 | 187 | Collagen I
(NM_053304.1) | Forward 5-CCA GCG GTG GTT ATG ACT TCA-3
Reverse 5-TGC TGG CTC AGG CTC TTG A-3 | 59 | 148 | Collagen III
(NM_032085.1) | Forward 5-GGTCACTTTCACTGGTTGACGA-3
Reverse 5-TTGAATATCAAACACGCAAGGC-3 | 59 | 201 | GAPDH
(NM_017008.4) | Forward 5-ACA GCA ACA GGG TGG AC-3
Reverse 5-TTT GAG GGT GCA GCG AAC TT-3 | 62 | 255 |
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