Ginkgolide B Suppresses TLR4-Mediated Inflammatory Response by Inhibiting the Phosphorylation of JAK2/STAT3 and p38 MAPK in High Glucose-Treated HUVECs

Figure 2

Ginkgolide B decreases PECAM-1 expression and the levels of IL-6 and MCP-1 in high glucose-treated HUVECs. The cells were treated with various concentrations of ginkgolide B for 1 h, and glucose or LPS was added for another 8 h. Protein expression was analyzed by Western blot. The column chart represents the density analysis from four independent experiments. (a) Ginkgolide B reduced PECAM-1 expression that was induced by high glucose. (b) Ginkgolide B decreased PECAM-1 expression that was induced by LPS. (c) Ginkgolide B reduced the levels of IL-6 in high glucose-treated cells. (d) Ginkgolide B reduced the levels of MCP-1 in high glucose-treated cells. ^#, significant difference between nonhigh glucose-treated cells and high glucose-treated cells (a) or between non-LPS-treated cells and LPS-treated cells (b); , significant difference compared non-ginkgolide B-treated cells and ginkgolide B-treated cells; , significant difference compared non-ginkgolide B-treated cells and ginkgolide B-treated cells.

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