Methylglyoxal Induces Changes in the Glyoxalase System and Impairs Glutamate Uptake Activity in Primary Astrocytes

Figure 2

Effect of MG on cell viability and survival in astrocytes and C6 cells. MTT reduction (a), NR incorporation (b), and PI incorporation (c) were performed to assess cellular viability after 24 h of MG exposure. In the MTT reduction and NR incorporation assays, each value represents mean ± standard error of four independent experiments performed in triplicate from astrocytes and C6 cells, assuming the control value as 100%. Values of fluorescence intensity for PI/field are expressed as the mean ± standard error of three independent experiments performed in triplicate. Data from each cell culture were analyzed by one-way ANOVA followed by Dunnett’s test, assuming . Significantly different from control.

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